methocult h4100 (methylcellulose medium)  (STEMCELL Technologies Inc)

Journal: iScience

Article Title: Lymphopoiesis is attenuated upon hepatocyte-specific deletion of the cytochrome c oxidase assembly factor Sco1

doi: 10.1016/j.isci.2025.112151

Figure Lengend Snippet:

Article Snippet: MethoCult H4100 (methylcellulose medium) , STEMCELL Technologies , Cat# 04100.

Techniques: Control, Enzyme-linked Immunosorbent Assay, Functional Assay, Western Blot, Recombinant, Protease Inhibitor, Diagnostic Assay, Staining, Software

Journal: Communications Medicine

Article Title: Enhanced fetal hematopoiesis in response to symptomatic SARS-CoV-2 infection during pregnancy

doi: 10.1038/s43856-023-00406-6

Figure Lengend Snippet: a , b Representative images of colonies grown for 14 days in methylcellulose from CD34+ HSPC sorted from COVID− and COVID+S samples. Photographs ( a ) and pictures taken at ×4 magnification ( b ) of representative 35-mm dishes are shown. Scale bar: 500 μm. c – e Quantification of colony-forming units for granulocytes, erythrocytes, monocytes, megakaryocytes (CFU-GEMM) ( c ), burst-forming units-erythroid and colony-forming units-erythroid (BFU-E/CFU-E) ( d ), and of colony-forming units for granulocytes and macrophages (CFU-GM) ( e ) in COVID− ( n = 3 patients) and COVID+S ( n = 3 patients) samples. Data are presented as means with SEM and individual values. Statistical analyses were performed with two-tailed unpaired t test ( d ) or two-tailed unpaired t test with Welch’s correction ( c , e ). ns not significant; * P value < 0.05. Source data are available in Supplementary Data .

Article Snippet: To perform colony cultures, 1000 cells/ml per condition of CD34+ progenitors were seeded in methylcellulose medium (H4100 Methocult media, StemCell Technologies) supplemented with 12.5% bovine serum albumin (Sigma-Aldrich) (neutralized with 7.5% sodium bicarbonate (Sigma-Aldrich)), 30% fetal bovine serum, 1 mM 2-mercaptoethanol (Sigma-Aldrich), 1% l -glutamine (Gibco), and in the presence of a cocktail of recombinant cytokines containing 1 U/mL EPO, 20 ng/mL TPO, 25 ng/mL SCF, 10 ng/mL IL-3, 10 ng/mL FLT3-L, 20 ng/mL G-CSF, 10 ng/mL IL-6 (all cytokines were purchased from Pepro Tech).

Techniques: Two Tailed Test